The influence of annealing of membrane electrode assembly (MEA) of a direct methanol fuel cell (DMFC) on the cell performance was investigated. The annealing was conducted at various temperatures of 110, 130, 150 and 200 °C. Annealing at 130 °C could produce highest proton conductivity of the recast Nafion binder and electrochemical active surface area of the electrode, leading to the highest cell performance.